Research on Fluorescence Detection System of Ca2+
A new fluorescence-micro-imaging system has been built using the microfluidic chip. By using this system fluorescence ratio of calcium ion (Ca2+) in cells were measured. Cell fostering, staining and driving can be implemented on the designed microfluidic chip. Then we make use of 340nm or 380nm laser to excite the calcium ion (Ca2+) and enable it emit fluorescence. The image of fluorescence was collected and analyzed with fluorescence-microimaging system. The concentration of calcium ion in cells can be calculated by using the fluorescence ratio method. Based on this new fluorescence-microimaging system, a new application domain was opened for microfluidic chip and a new detection means was developed for measuring calcium ion (Ca2+) in cells.
microfluidic chip signal analysis the concentration of calcium ion cell detection microscope fluorescence ratio
Bao Liu Sixiang Zhang Wei Zhou Yinxia Chang
Research Institute of Modern Measurement & Control Technology Hebei University of Technology Tianjin, China
国际会议
厦门
英文
106-108
2010-10-26（万方平台首次上网日期，不代表论文的发表时间）