Construction of L-threonine Genetic Engineering Strain by Red Recombination

摘要：

The threonine genetic engineering strain was constructed by a strategy of cut off branch metabolic pathways and overexpression thr operon in E. coli ITHR. The metA gene was knocked out by Redrecombination systems and E. coli ITHR△metA was constructed respectively. The pYOOl containing thr operon was transformed into the mutant strain by electroporation. Fed-batch cultures of genetic engineering strain were carried out in 5 L fermentors and the threonine concentration was determined by HPLC. The results showed that E. coli ITHR carrying pYOOl could accumulate 5.55±0.51 g/L threonine. When metA was knocked out respectively, the threonine productions reached 9.77±1.83 g/L. The productivity of L-threonine by E. coli ITHR can be enhanced through the knocking out the gene of the key enzymes in branch metabolic pathways, including the methionine synthesis pathway.

关键词： Red recombination Gene Knockout L-Threonine Fermentation

作者: YAN Ji-ai

作者单位: College of Biotechnology Tianjin University of Science & Technology Tianjin 300457, China

会议类型: 国际会议

会议名称: 2010 Second Asia-Pacific Conference on Information Processing(2010年第二届亚太地区信息处理国际会议 APCIP 2010)

会议地点: 南昌

会议语种:英文

页码: 292-294

在线出版日期: 2010-09-17（万方平台首次上网日期，不代表论文的发表时间）