Experimental Study for Automatic Colony Counting System Based on Image Processing
Colony number in many colony calculating experiments is counted by manual method at present, therefore it is difficult to get the result quickly and accurately when the number of experimental sample is large. A new automatic colony counting system was developed, which makes use ol image-processing technology to feasibly count white bacterial colonies in clear plates according to the RGB color theory. A way of obtaining optimal threshold of segment image was obtained based upon a lot of experiments on the distribution ol the colonys chromatic value. It has been proved that the method greatly improves the accuracy and efficiency of the colony counting and the counting result is not affected by inoculation method, shape or size of the colony. It is revealed that automatic detection of colony quantity using imageprocessing technology could be an effective way.
colony counting image processing optimal chromatic value automatic detection
SHEN Wei-zheng ZHAO Jie WU Ya-chun ZHENG Hui
Engineering College, Northeast Agricultural University Harbin, China Northeast Agricultural University Harbin, China
国际会议
太原
英文
612-615
2010-10-22（万方平台首次上网日期，不代表论文的发表时间）