Mitochondrial DNA D-loop Analysis of Animal Hair Fibres for Species Identification in Textiles
There is an increase in demand for animal natural fibres in recent years for the production of high quality textiles. These fibres are expensive because of limited feedstock and less fibres production. Hence to keep up the demand these fibres are adulterated with less expensive fibres viz. wool to give special effects to the fabric. This leads to false declaration of the fibres ultimately causing heavy financial loss. To keep control on false declaration, there is a need for fibre identification and to ascertain blend composition. Scanning Electron Microscopy is usually used for fibre analysis but the method has some limitations as it is time consuming, expensive and the reliability of results depended on the expertise of the microscopist. There is a need for reliable and economical method to characterize these fibres and to study composition of each animal fibre in blends. The aim of the present study was DNA analysis of animal natural fibres in untreated as well as the blends. We extracted mitochondrial DNA (mtDNA) from animal hair shafts of sheep, goat, camel, rabbit and the blend of all samples by phenol/chloroform/Isoamyl alcohol method and amplification of D-loop by using specific primers.
speciality fibres d-loop mitochondrial dna polymerase chain reaction species - specific primer
RANE Priyanka P. SAHASRABUDHE Abhijit V. BARVE S.S.
KET’s V.G.Vaze College of Arts, Science and Commerce, Mithagar Road, Mulund (E), Mumbai- 81.Maharash Research Associate Reliable Analytical Laboratories, Bhiwandi, Thane, Maharashtra, India KET’s V.G.Vaze College of Arts,Science and Commerce, Mithagar Road, Mulund (E), Mumbai- 81.Maharasht
国际会议
The 12th International Wool Research Conference(第十二届国际羊毛会议12th IWRC)
上海
英文
944-945
2010-10-19（万方平台首次上网日期，不代表论文的发表时间）