Advances in Fibre Proteomics: Evaluation of Fibrous Protein Damage and Protection Using Molecular Markers
Protein degradation is a critical consideration in the quality, performance and appearance of proteinaceous materials such as wool, silk, human hair and skin. Fibrous protein protection and repair technologies are becoming increasingly important in the formulation of functional treatments for these substrates. Evaluation of protein oxidative damage has traditionally been undertaken at a holistic level. However, for targeted design and validation of next generation treatment technologies, it is important that robust and sensitive approaches for evaluating protein damage at the molecular level are developed. We here present an overview of the development and utilisation of a peptide-based molecular marker approach for profiling and tracking oxidative damage in complex protein systems. Redox proteomic approaches were applied to characterise oxidative modification at the protein primary level in intermediate filament proteins. Specific photoproducts were tracked across varying damage conditions. Molecular markers were then utilised to profile damage within whole wool both with and without application of a protective surface treatment, demonstrating the efficacy and sensitivity of this approach for evaluating protein protection.
proteomics protein keratin damage marker
DYER Jolon GROSVENOR Anita CORNELLISON Charisa CLERENS Stefan DEB CHOUDHURY Santanu
Protein Quality and Function, Lincoln Research Centre, AgResearch Ltd, New Zealand
国际会议
The 12th International Wool Research Conference(第十二届国际羊毛会议12th IWRC)
上海
英文
980-983
2010-10-19（万方平台首次上网日期，不代表论文的发表时间）