Ramie Soil Metagenomic Library Construction and Functional Screening

摘要：

Metagenomics method is used to explore the diversity of uncultured microbial communities and obtain novel environmental DNA without any cultivation. The aim of the study is to promote analysis of unknown microorganism from environment and ecological system of marine sediments. About 5×104 clones have been produced totally in the library. The library was screened for both xylanase activity and pectinase activity, and 18 positive clones were isolated, bypassing the traditional microbiological research methods.

关键词： degumming metagenomics fosmid library xylanase pectinase

作者: LI Lijiong CHEN Yangdongi DING Ruoyao SHAO Zhiyu CAO Zhangjun ZHANG Xingqun

作者单位: Key Laboratory of Science & Technology of Eco-Textile (Donghua University/Jiangnan University),Minis College of Chemistry, Chemical Engineering & Biotechnology, Donghua University, Shanghai 201620, P.R

会议类型: 国际会议

会议名称: The 12th International Wool Research Conference(第十二届国际羊毛会议12th IWRC)

会议地点: 上海

会议语种:英文

页码: 989-992

在线出版日期: 2010-10-19（万方平台首次上网日期，不代表论文的发表时间）