Acute Isolation of Neurons Suitable for Patch-Clamping Study from Frontal Cortex of Mice
A method was described for the acute isolation of neurons suitable for patch-clamping study from the frontal cortex of 7-10-d-old Kunming mice by a combination of mechanical and enzymatic means. Using inverted microscope and whole-cell configuration of patch-clamp technique, the morphological and electrophysiological properties of cortical neurons were studied respectively. It was shown that the enzymatically isolated neurons had plump profile, smooth surface, strong aureole and long survival time, met the electrophysiological requirements, and exhibited the whole-cell transmembrane currents, voltage-gated sodium and potassium currents. The experiment proves that this method is simple, efficient, reliable and utility. The dissociated cortical neurons could be obtained and applied to patch-clamping study, which has reference value for studying the effects of physiology, pathology, pharmacology and physical factors on the ion channels of the cortical neurons of mice.
frontal cortex neuron acute isolation mice patch-clamp technique
Yuan-yuan Li Li-jun Cheng Gang Li Ling Lin Dan-dan Li
College of Precision Instruments and Opto-Electronics Engineering,Tianjin University, Tianjin, China College of Precision Instruments and Opto-Electronics Engineering,Tianjin University, Tianjin, China
国际会议
无锡
英文
611-617
2010-09-17(万方平台首次上网日期,不代表论文的发表时间)