IN VITRO AND IN VIVO GENE DELIVERY USING POLYETHYLENIMINE-POLY(HYDROXYETHYL GLUTAMINE) AS A NON-VIRAL CARRIER

摘要：

Polyethylenimine-poly(hydroxyethyl glutamine) copolymer (PEI-PHEG) could effectively form positively charged nanoparticles with pDNA. The PEI-PHEG copolymer exhibited much lower cytotoxicity and higher gene transfection efficiency than PEI-25k with different cell lines in vitro. PEI-PHEG/pDNA complex demonstrated excellent transfection efficiency in animal tests. PEI-PHEG could efficiently deliver siRNA to cells to silence the target gene. It could also complex vascular endothelial growth factor siRNA (VEGF siRNA) to achieve the aim of tumor therapy.

关键词： hydrophilic gene delivery RNA interference VEGF tumor therapy

作者: Jie Chen Huayu Tian Arihiro Kano Atsushi Marayama Xuesi Chen Tae Gwan Park

作者单位: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Institute for Materials Chemistry and Engineering, Kyushu University, Fukuoka 812-8581, Japan Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-7

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 330-332

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）