DENDRIMER MODIFIED MAGNETIC IRON OXIDE NANOPARTICLE/DNA/PEI TERNARY COMPLEXES: A NOVEL STRATEGY FOR MAGNETOFECTION

摘要：

Polyamidoamine dendrimer modified magnetic iron oxide nanoparticle/DNA/PEI (25 kDa) ternary complexes were used for the magnetofection of mammalian cells. The results indicated that the transfection efficiencies of COS 7 cells with ternary magnetoplexes significantly increased when a magnetic field was applied, especially in the presence of 10％ serum. Further evidence from Prussian blue staining of iron inside the cells and intracellular trafficking of Cy-3 labeled DNA demonstrated that the magnetic field quickly gathered the magnetoplexes to the surface of target cells and enhanced the uptake of the ternary magnetoplexes by the cells. This represents a novel strategy for polycation-based in vitro gene delivery enhanced by a magnetic field.

关键词： iron oxide nanoparticles dendrimer polyethylenimine magnetofection gene delivery

作者: Wen-Ming Liu Ya-Nan Xue Wen-Tao He Ren-Xi Zhuo Shi-Wen Huang

作者单位: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry,Wuhan University, Wuhan 430072, China

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 378-380

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）