BIOREDUCIBLE CATIONIC MICELLE/DNA MULTELAYERED FILMS FOR LOCALIZED GENE DELIVERY

摘要：

Multilayered films composed of bioreducible cationic micelles, formed from amphiphilic disulfide-containing poly(amidoamine)s, and DNA were prepared via layer-by-layer assembly for localized gene delivery. The thickness of cationic micelles/DNA films with 10 bilayers increased with increasing the grafting degree of alkyl groups in poly(amidoamine)s. Meanwhile, films with 10 bilayers prepared from bioreducible polycations with 28％ of alkyl side chains, showed the fastest release of DNA in the presence of 2.5 mM glutathione and highest transfection efficiency when 293T cells were cultured on the surface of films.

关键词： multilayered films bioreducible cationic micelles disulfide transfection

作者: Na Peng Ya-Nan Xue Xi-Ming Xia Shi-Wen Huang Ren-Xi Zhuo

作者单位: Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan 430072, China

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 392-394

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）