PREPARATION AND CHARACTERIZATION OF GUANIDINATED N-3-AMINOPROPYL METHACRYLAMIDE-N-2-HYDROXYPROPYL METHACRYLAMIDE COPOLYMERS AS GENE DELIVERY CARRIERS

摘要：

N-3-guanidinopropyl methacrylamide-N-2-hydroxypropyl methacrylamide copolymers (GPMA-HPMA) synthesized via radical copolymerization of N-3-aminopropyl methacrylamide (APMA) and N-2-hydroxypropyl methacrylamide (HPMA) followed by guanidination were designed to develop novel non-viral gene carriers with cell membrane-penetrating properties. Synthesized GPMA-HPMA copolymers complex with pDNA completely at charge ratios no more than 1, and the positively charged complexes have diameters about 200 nm. Furthermore, the guanidinated copolymers showed significantly decreased cytotoxicity, efficient trans fection at low charge ratios with pDNA, and good nuclear localization ability.

关键词： cellular uptake guanidination GPMA-HPMA copolymer nuclear localization

作者: Zhu Qin Wei Liu Liang Guo Xinsong Li

作者单位: Biomaterials and Drug Delivery Laboratories, and School of Chemistry and Chemical Engineering,Southeast University, P.O.Box 211189, Nanjing, China

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 398-400

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）