ANTISENSE OLIGODEOXYNUCLEOTIDE COMPLEXATION WITH PHOSPHORYL-CHOLINE DIBLOCK COPOLYMERS AND ITS EFFECT ON CELL TRANSFECTION

摘要：

MPC30-DEA70/c-myc AS-ODN complexes were formed at different N/P ratios and transferred into HeLa cells. The complexes were studied using gel electrophoresis, fluorescent microscopy, FCM, and MTT assay. It was shown that the novel cationic phosphorylcholine copolymer MPC30-DEA70 could load and transport human c-myc antisense oligonucleotides. The transfection efficiency of the complexes and cell growth inhibition were significantly enhanced with increasing of N/P ratios.

关键词： PC polymer antisense oligodeoxynucleotide cell transfection gene delivery

作者: Tiantian Sun Zhuoqi Zhang Xichuan Cao Min Zhang Xia Zhao Qi Zhang Minmin Chen

作者单位: School of Materials Science and Engineering, China University of Mining and Technology, Xuzhou 22111 Department of Cardiology, the Affiliated Hospital of Xuzhou Medical College, Xuzhou 221002, China

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 404-406

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）