IMMOBILIZATION OF GENE VECTORS ON BISPHOSPHONATE-MEDIATED GENE-ELUTING METAL STENTS USING ANTIBODY FOR LOCALIZED GENE DELIVERY

摘要：

A metal stent that could elute plasmid DNA (pDNA) for substrate-mediated gene transfection was fabricated by first coating with polyallylamine bisphosphonate (PAA-BP) and subsequent deposition of pDNA. To create a pDNA coating layer on the stent surface, anti-DNA antibody was chemically coupled on the PAA-BP coating. PAA-BP was conjugated to IgM by covalent cross-linking with N-succinimidyl-3(2-pyridyldithio) propionate (SPDP). In cell culture studies, green fluorescent protein (GFP)-transfected cells were only found on the stent, which demonstrated high localization and efficient gene delivery.

关键词： gene vector plasmid DNA local gene delivery stent

作者: Yong Wang Mei Yu Linhua Zhang Cunxian Song Ivan S Alferiev Robert J.Levy

作者单位: Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical Co Childrens Hospital of Philadelphia, Abramson Research Building, Philadelphia, United States

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 407-409

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）