POLY(ETHYLENE OXIDE) GRAFTED WITH LOW MOLECULAR WEIGHT POLYETHYLENIMINES FOR NON-VIRAL GENE TRANSFER

摘要：

A novel gene delivery system based on poly(ethylene oxide) (PEO) grafted with 1.8 kDa branched polyethylenimine (PEI) (PEO-g-PEI) was developed. Three PEO-g-PEI copolymers, i.e. PEO(13k)-g-PEI10, PEO(24k)-g-PEI10, and PEO(13k)-g-PEI22 were synthesized. All copolymers were able to condense DNA into small-sized polyplexes (<200 nm) at and above an N/P ratio of 10. The polyplexes of PEO-g-PEI revealed enhanced stability in 150 mM NaCl. Interestingly, polyplexes of PEO(13k)-g-PEI10 while maintaining a low cytotoxicity showed remarkably higher transfection activities in 293T cells as compared to the 25 kDa PEI control.

关键词： polyethylenimine poly(ethylene oxide) graft copolymers gene delivery

作者: Meng Zheng Lei Zhou Ru Cheng Fenghua Meng Rui Peng Zhiyuan Zhong

作者单位: Biomedical Polymers Laboratory and Jiangsu Key Laboratory of Organic Chemistry, College of Chemistry Institute of Functional Nano & Soft Materials (FUNSOM), Soochow University, Suzhou 215123,China

会议类型: 国际会议

会议名称: The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)

会议地点: 苏州

会议语种:英文

页码: 431-433

在线出版日期: 2010-09-14（万方平台首次上网日期，不代表论文的发表时间）