SURFACE-FUNCTIONALIZED SILK FIBROIN FILM WITH CONTROLLABLE CELL ADHESION BY SURFACE-INITIATED ATOM TRANSFER RADICAL POLYMERIZATION
Surface-initiated atom-transfer radical polymerization (ATRP) was employed to tailor the functionality of silk fibroin (SF) film surface in a well-controlled manner. Functional polymer brushes of N-isopropylacrylamide (NIPAAm) and 2-hydroxyethyl methacrylate (HEMA) were prepared via surface-initiated ATRP from the SF films. Surface cultures of the 3T3 cell line on the functionalized SF films were evaluated. At 37 °C, the seeded cells adhered, spread, and proliferated on the SF films grafted by P(NIPAAm) and P(HEMA)-b-P(NIPAAm). Below the LCST, the cells detached from the SF-g-P(NIPAAm) surface spontaneously. SF films grafted with P(HEMA) and P(NIPAAm)-b-P(HEMA) brushes showed similar cell attachment/detachment with that of P(HEMA) modified SF films, respectively. These functionalized SF films can be potentially useful as SF-based biomedical materials, such as wound dressing, wound healing and cell cultivation substrates.
silk fibroin ATRP PNIPAAm PHEMA cell detachment
Zhengbiao Zhang Fujian Xu Xiulin Zhu Entang Kang KoonGee Neoh
Provincial Key Laboratory of Organic Synthesis, College of Chemistry, Chemical Engineering and Mater State Key Laboratory of Chemical Resource Engineering College of Materials Science and Engineering, Provincial Key Laboratory of Organic Synthesis, College of Chemistry, Chemical Engineering and Mater Department of Chemical and Biomolecular Engineering, National University of Singapore, Kent Ridge 11
国际会议
The First Symposium on Innovative Polymers for Controlled Delivery(新型高分子材料与控制释放国际会议 SIPCD 2010)
苏州
英文
596-598
2010-09-14（万方平台首次上网日期，不代表论文的发表时间）