The Effects of Transport Stress on Some Antioxidative and Immune Parameters in Cynoglossus Semilaevis G(u)nther Fry
Cynoglossus semilaevis Gunther fries (4.93±0.96 g) were subjected to 11 hours practical transport stress to determine the antioxidative and immune responses post-transport in this species. Ten fish were sampled randomly pre-transport and at 0, 4, 12, 24, 48 and 96 h post-transport, respectively. After transport stress, the superoxide dismutase (SOD), lysozyme (LZM) and complement C3 decreased sharply by 60％, 82％ and 87％ at 0 h post-transport, respectively. The SOD and LZM recovered to pre-transport levels 96 h post-transport. The complement C3 maintained at low level during post-transport observation, being 75％ lower than that of pre-transport. Transport caused a transient decrease in malondidehyde (MDA) during 12 hours post-transport. The catlase (CAT) declined significantly, reached the lowest value at 12 h (decreased by 77％) post-transport, and restored to pre-transport level at 48 h post-transport. The reactive oxygen species (ROS) peaked at 0 h post-transport (increased by 2.4-fold) then decreased gradually, restored to pre-transport level at 48 h post-transport. The glutathione (GSH) rose continuously, peaked at 48 h post-transport (increased by 2.7-fold). The GSH may be an offset of decreased SOD and CAT activities, and played an important role in reducing oxidative stress. Suppressed SOD, CAT, LZM and complement C3 levels following transport stress suggested a possible increased susceptibility to bacterial infections after stress. At least 96 hours of recovery time was needed for C. semilaevis fry after long-time commercial transport.
Transport stress Antioxidation Immunity Cynoglossus semilaevis Gunther
WANG Qingkui CHEN Chengxun SUN Xueliang XING Kezhi
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, 5 Yu Shan Road, Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Science, Tianjin Agric
国际会议
China Agriculture System Engineering Society 2010(2010年中国农业系统工程学术年会)
威海
英文
304-309
2010-07-29（万方平台首次上网日期，不代表论文的发表时间）