Flow Cytometric Analysis of Effects of Copper Sulfate on Hemocyte Defence Functions of the Penaeid Shrimp, Penaeus Vannamei
The acute effects of copper sulfate on the immune parameters of hemocyte from Penaeus vannamei were determined with the flow cytometric (FCM) analysis. For the immune parameters assays, total haemocyte count (THC), apoptosis percentage, respiratory burst activity, and phagocytic activity were used as indicators. P. vannamei following 48 h exposure to 1.0 mg 1-1 Cu2+ did not affect its THC, but decreased significantly its THC following 12 h exposure to 8 mg 1-1 Cu2+. P. vannamei following 24h exposure to Cu2+ at 16mg 1-1 significantly stimulated the production of superoxide anion. Phagocytic activity decreased in P.vannamei following 24h exposure to 1, 4, 8 and 16 mg 1-1 Cu2+. The cell apoptosis study showed that the following 48 h exposure to 4-16 mg 1-1 Cu2+ can significantly induce the haemocyte apoptosis. This study for the first time led to an analysis of acute effects of copper sulfate on hemocyte defence functions of the penaeid shrimp with the FCM. It is suggested that acute exposure to lethal levels of copper may be one of the factors responsible for the decline of shrimp stocks in the Pearl River estuary and other coastal waters of southern China.
Flow cytometry Copper sulfate Hemocyte Defence functions Penaeus vannamei
SUN Jingfeng CHEN Chengxun WANG Anli XIAN Jianan
Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South Tianjin Key Lab of Aqua-Ecology and Aquaculture, Department of Fisheries Science, Tianjin Agricultur Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South
国际会议
China Agriculture System Engineering Society 2010(2010年中国农业系统工程学术年会)
威海
英文
317-323
2010-07-29（万方平台首次上网日期，不代表论文的发表时间）