Dietary Compound Chinese Herbal Medicine Enhanced Phagocytosis and Lysozyme Activity in Litopenaeus vannamei
To investigate dietary compound Chinese herbal medicine (CCHM) on phagocytosis and lysozyme activity in Litopenaeus vannamei, four CCHMs were prepared. CCHM-Ⅰ was composed of radix Astragalus, radix Atractylodis Macrocephalae, radix Glycyrrhizae and rhizoma Alisma. CCHM-Ⅱ was composed of rhizoma Picrorhizae, Scrophularia ningpoensls and loosestrife. CCHM-Ⅲwas composed of Lonicera japonica and Gardenia jasminoides. CCHM-Ⅳwas composed of Rheum Rhaponticum, Scutellaria baicalensis and Phellodendron chinense Schneid. Each CCHM was supplemented in basal diet at three levels (8‰, 11‰ and 15‰). Control group was fed diet without CCHM. Each diet was fed to nine groups of shrimp (triplicate in each level, initial body length 10.4±1.2 cm) for 40 days. Phagocytic rate (PR) and phagocytic index (PI) in haemolymph and lysozyme activity in hepatopancreas and muscle was assayed after 40-day feeding trial. Results showed that PR and PI were enhanced in CCHM supplemented groups in the following order: 8‰>11‰>15‰>control groups. PR and PI in CCHM groups showed no significant difference among four CCHM supplement groups. Lysozyme activity in hepatopancreas in CCHM-Ⅰ (11‰), CCHM-Ⅱ (8‰ and 15‰) and CCHM-Ⅲ (15‰) groups was higher than that in control groups,Lysozyme activity in muscle in CCHM-Ⅰ (8-15‰), CCHM-Ⅲ (8‰ and 15‰) was higher than control. No significant difference in lysozyme activity was observed in shrimp fed CCHM-Ⅳ. These results indicated that the four types of CCHM enhanced nonspecific immunity of Litopenaeus vannamei in varying degrees.
Litopenaeus vannamei Compound Chinese herbal medicine Phagocytic ability Lysozyme activity
CHEN Chengxun WANG Qingkui BAI Dongqing XING Kezhi GUO Yongjun
Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Science, Tianjin Agricultural University, 22 Jin Jing Road, Tianjin 300384, PR China
国际会议
China Agriculture System Engineering Society 2010(2010年中国农业系统工程学术年会)
威海
英文
324-327
2010-07-29（万方平台首次上网日期，不代表论文的发表时间）