Effect of Injected Angelica Polysaccharide on Non-specific Immunity in Grouper, Epinephelus Malabaricus
A preliminary study was conducted to determine the effect of injected Angelica sinensis polysaccharide (ASP) on non-specific immunity of Epinephelus malabaricus.The ASP was extracted from radix Angelica sinensis in the following procedure:boiled the radix powder in water,deposited polysaccharide dissolved in water extract with 80％ ethanol after filtration,deproteinized with Sevag reagent several times after decolorization with ethanol,and lyophilized.The ASP powder was dissolved in PBS and i.p.injected to fish (body weight 80.2±5.1 g) at three dosages (5,15 and 30 mg ASP/kg body weight).Control groups were i.p.injected with PBS.Twenty fish at each dosage were sampled randomly on day 1,3,5 and 7 post-injection.Serum lysozyme activity in ASP injected groups was enhanced on day 3,5 and 7 and statistically significant in group 15 and 30 mg ASP/kg body weight.Blood phagocytic ratio (PR) was enhanced in ASP injected fish.Serum albumin/globulin (A/G) ratio decreased in ASP injected fish.A negative correlation was observed between A/G ratio and ASP injection dosage.These results indicated that ASP enhanced innate immunity of E.malabaricus.
Epinephelus malabaricus Angelica sinensis polysaccharide I.p.injection Innate immunity
WANG Qingkui CHEN Chengxun ZHAO Haiyun XING Kezhi
The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China,5 Yu Shan Road, Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Department of Fishery Science, Tianjin Agric
国际会议
China Agriculture System Engineering Society 2010(2010年中国农业系统工程学术年会)
威海
英文
328-332
2010-07-29（万方平台首次上网日期，不代表论文的发表时间）