Expression of hepatitis C virus F protein leads transfected cell to transformation

摘要：

In this study, we aim to investigate the role of HCV F on the cellular transformation. HCV F gene was amplified using PCR and cloned into the eukaryotic expression plastnid pcDNA3. 0. NIH3T3 cells were transfected with pcDNA3-F plasmid using Lipofectamine 2000 transfection reagent. Anchorage-independent growth in soft agar was then carried out. Transfected cell lines were obtained from experimental transfections, which showed anchorage-independent growth in soft agar. In conclusion, overexpression of the F protein resulted in an increased proliferation rate in NIH3T3 cells, the core protein expression also did induce cellular transformation.

关键词： Hepatitis C virus (HCV) F protein cellular transformation

作者: Kong Jing Deng Xiao-zhao Chu Chun-li Zhang Yun Wang Zhong-chan Li Chun-lei

作者单位: Huaiyin Institute of Technology, 1 Mei-cheng Road, Huaian, 223300, China,Medical Institute of Nanjin Medical Institute of Nanjing Army, 293 Zhong-shan East Road, Nanjing, 210002, China

会议类型: 国际会议

会议名称: International Symposium on Pharmacogenomics and the Regulation of Drug Metabolism Enzymes and Genes(药物基因组学和药物代谢酶及基因调控国际研讨会)

会议地点: 南京

会议语种:英文

页码: 40-42

在线出版日期: 2010-05-12（万方平台首次上网日期，不代表论文的发表时间）