HCV F protein triggers IL-10 production in peripheral blood monouclear cells

摘要：

Researchers reported a novel F protein, an alternative reading frame protein expressed from the HCV core coding sequence. F protein can induce humoral immunity in HCV infected patients. In this study, we aim to investigate whether this protein induce specific cellular immunity responses. Monocytes were separated from peripheral blood of healthy and HCV infected individual by centrifugation on FicollHypaque density gradient. PBMCs were stimulated with HCV F protein. Supernatants were collected and analyzed by ELISA. HCV F protein induced PBMCs from HCV patients only to produce IL-10, but not TNF-α cytokine. However, HCV F protein could trigger IL-10 and TNF-α cytokines in PBMCs from healthy donors.

关键词： Hepatitis C virus (HCV) F protein IL-10 peripheral blood monouclear cells (PBMC)

作者: Kong Jing Deng Xiao-zhao Chu Chun-li Zhang Yun Wang Zhong-chan Li Chun-lei Wei Juan

作者单位: Medical Institute of Nanjing Army, 293 Zhong-shan East Road, Nanjing, 210002, China,Huaiyin Institut Medical Institute of Nanjing Army, 293 Zhong-shan East Road, Nanjing, 210002, China

会议类型: 国际会议

会议名称: International Symposium on Pharmacogenomics and the Regulation of Drug Metabolism Enzymes and Genes(药物基因组学和药物代谢酶及基因调控国际研讨会)

会议地点: 南京

会议语种:英文

页码: 43-45

在线出版日期: 2010-05-12（万方平台首次上网日期，不代表论文的发表时间）