Analysis of DNA mismatch repair activity in live cells

摘要：

Loss of DNA mismatch repair (MMR) function leads to the development and progression of certain cancers. Currently, assays for DNA MMR activity involve the use of cell extracts and are technically challenging and costly. In this study, we develop a convenient spectrometric assay system for specific and quantitative measurement of DNA mismatch repair activity intracellularly. A G-T mismatch was introduced to the upstream region of firefly luciferase coding sequence in the pGL3-Control plasmid. Only if the G:T mismatches were repaired to G:C, will luciferase be expressed in transfected cells. By measuring luciferase activity, it is simple and convenient to assay intracellular DNA mismatch repair activity.

关键词： DNA mismatch repair intracellular luciferase

作者: Li Shiying Zhang Xin Qiu Fan Hua Zi-Chun

作者单位: The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing, 210093, China

会议类型: 国际会议

会议名称: International Symposium on Pharmacogenomics and the Regulation of Drug Metabolism Enzymes and Genes(药物基因组学和药物代谢酶及基因调控国际研讨会)

会议地点: 南京

会议语种:英文

页码: 46-50

在线出版日期: 2010-05-12（万方平台首次上网日期，不代表论文的发表时间）