Expression and Bioactivity of Recombinant Human Cytoglobin
To investigate the function and pharmaceutical application of cytoglobin. Cytoglobin cDNA was cloned from cDNA library of HepG2 cell by RT-PCR and then subcloned into expression vector pET28a. The recombinant expression vector was transformed into the E. coli BL21, the genetic engineering strain pET28a-rhCYGB/BL21 was constructed, the strain was fermentated in large scale (5L). The expression of rhCYGB was induced by IPTG at concentration of 0. 5 mM. The purified rhCYGB (95％) was obtained through several purification steps. The in vitro and in vivo experiments demonstrated that recombinant human CYGB has antioxidant activity and could be a potential biotech drug for treatment of liver fibrosis.
cytoglobin stellate cell activation-associated protein antioxidation antifibrosis
Weijuan Tao Xiaojian Jia Wei Wei Dongya Chen Zhenwang Jie Wutong Wu Yubin Zhang
School of Life Science and Technology, China Pharmaceutical University, Nanjing, China
国际会议
南京
英文
54-58
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）