Effect of cholesterol myristate on proliferation of mesenchymal stem cells is via retinoic acid receptor
Cholesterol myristate, an active compound commonly found in Plastrum testudinis in traditional Chinese medicine, promoted the proliferation of mesenchymal stem cells (MSCs) recently demonstrated by us. The present study is further designed to determine the molecular mechanism by which cholesterol myristate exerts proliferative effects. MSCs were stimulated with different dose of cholesterol myristate for 3 days. retinoic acid receptor-α (RARα) mRNA was examined by in situ hybridization and RT-PCR. Both analyses demonstrate an increase in RARα mRNA expression. Furthermore, Western blot analysis revealed that cholesterol myristate significantly increase RARα levels in a time-and dose-dependent manner. Detection of the number of RARα-positive cells by flow cytometry and immunofluorescent staining show that cholesterol myristate increases the number of RARα-positive cells in a dose dependent manner. Taken together, our results indicate that the mechanism of cholesterol myristate action may be associated with the up-regulation of RARa. Moreover, a RAR-selective antagonist counteracts the effects of cholesterol myristate on MSCs proliferation. This study is to provide evidence that the effect of cholesterol myristate involves retinoic acid receptor on mesenchymal stem cells proliferation.
cholesterol myristate retinoic acid receptor mesenchymal stem cells
Dong-Feng Chen Jia-hui Cao Zi-Chun Hua
State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry,Nanjing University, Department of Anatomy, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry,Nanjing University,
国际会议
南京
英文
65-70
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）