Expression, purification and characterization of ras oncogene (Bras2)from silkworm (Bombyx mori)
Ras oncogene (Bras2) from silkworm pupae may belong to the Ras superfamily. The recombinant His-tag BmBras2 was expressed successfully in E. coli Rosetta (DE3) induced by IPTG. Western blot and real time PCR analyses showed that BmBras2 is expressed during four developmental stages, and the expression level of BmBras2 is highest in pupae and lower in the fifth instar larvae, moth and nascent egg; BmBras2 is expressed in all eight tissues, and it is highly expressed in head, intestine and epidermis. The result of subcellular localization indicates that BmBras2 mainly localize at the nuclei of Bm5 cells, partly at cytoplasms, but not at plasma membranes.
Bombyx mori ras oncogene (Bras2) expression analysis subcellular location
Zhuang Wen-Hua Zhang Xue-Fang Lv Zheng-Bing Zhang Yao-Zhou
Institute of Biochemistry, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou,310018, China
国际会议
南京
英文
80-83
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）