Development and application of a neutralization assay based on pseudotyped virus for anti-HIV-1 drug discovery
HIV-1 envelope glycoprotein (Env) plays major roles for HIV-1 attachment and entry, thus a major target for anti-viral drug development. Here we described the optimization and validation of an assay that measures HIV-1 neutralization in GHOST (3) X4/R5 cells as a function of reductions in luciferase (Luc) reporter gene expression after a single round of Env-pseudotyped viruse infection. We determined the Env: backbone plasmid ratios in preparing pseudovirons in 293T cells, and tested the inhibitory activities of a number of HIV-1 attachment and entry inhibitors, including protein, small molecular compound and total IgG prepared from HIV-infeced patient sera,. The results showed that the pseudovirus assay was a sensitive method for measuring inhibitory activities of various anti-viral agents with distinct working mechanisms. The system is particularly suitable for analysis and evaluation of inhibitors that block HIV-1 attachment and entry.
HIV-1 neutralization assay pseudotyped virus anti-HIV-1 drug
Chu Ying Song Hongyong Wang Qin Yuan Zhongping Wu Zhiwei
Center for Public Health Research, Medical School, Nanjing University, 210093, China
国际会议
南京
英文
84-87
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）