Down regulation of CCR5 by asymmetric siRNA duplex-mediated RNA interference
CCR5, a chemokine receptor, is a critical molecule serving as a co-receptor for HIV infection. CCR5 has been studied as a potential target for inhibiting HIV-1 infection, and studies showed that it could be efficiently down regulated by small interfering RNAs (siRNA) in a sequence-specific manner. However, the current standard synthetic 19 + 2 siRNA structure exhibited several sequenceindependent, nonspecific effects. To reduce off-target silencing, a series of optimized CCR5 sequencespecific asymmetric siRNAs were designed and synthesized. 48hours after transfection, the mRNA of CCR5 was analyzed by qRT-PCR, and the cellular CCR5 expression was analyzed by flow cytometry. Compared with symmetric siRNA, some of the asymmetric siRNA (asiRNA) with a shorter sense strand exhibited similar suppressive activity on the expression of CCR5. These results suggested that asiRNA is a potential approach for HIV therapeutic or preventative applications.
asiRNA RNAi CCR5 HIV
Huijuan Ge Zhiwei Wu
Center for Public Health Research, Medical School, Nanjing University, Nanjing, 210093, China
国际会议
南京
英文
96-99
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）