Effects of fish cytochromes P450 inducers and inhibitors on difloxacin N-demethylation in kidney of Chinese idle (Ctenopharyngodon idellus)
Cytochromes P450 (CYPs) play key roles in drug metabolism which are widely distributed in kidney in aquatic organisms. In order to supply useful information on CYP(s) characterization for DIF N-demethylation, the present study assessed the effects of fish potent CYP inducers and inhibitors on DIF N-demethylation and the inductive and inhibitive enzyme kinetics in kidney of Chinese idle (Ctenopharyngodon idellus) by reversed-phase high-performance liquid chromatography (RP-HPLC). Results demonstrated that the amounts of SAR formation pretreated by β-naphthoflavone (BNF) increased by 1.1 fold and ornaphthoflavone (ANF) inhibited SAR formation level by 0. 6 fold at the third day. Enzymatic parameters Vmax and Clint of DIF N-demethylase were increased by 0. 56 and 0. 38 fold due to β-naphthoflavone (BNF) pretreatment. BNF and ANF are the separate typical inducer and inhibitor for CYP1A in fish. Thus, we suggest that CYP1A may be responsible for DIF N-demethylation in kidney.
cytochromes P450 difloxacin inhibition inductin kinetic
Yu Lingzhi Yang Xianle
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, 201306, China
国际会议
南京
英文
109-113
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）