Ramulus dnnamomi (L.) inhibit matrix metalloproteinase activities and the motility of HT1080 cells
Traditional Chinese medicines (TCM) have been widely used and achieved great success in Chinese clinic. However, the therapeutic mechanism was mostly unclear. Ramulus Cinnamomi is used in many TCM formulations for the treatment of arthralgia or numbness of the extremities, relieving phlegm, amenorrhea and menorrhagia. To examine the biological activities of Ramulus Cinnamomi on a molecular level, we investigated the inhibition of Ramulus Cinnamomi extracts against matrix metalloproteinases (MMPs). The water extract of Ramulus Cinnamomi was prepared followed by several organic solvent extractions. The primery water extract, n-butyl alcohol extract and final water extract can effectively inhibit MMP-2, MMP-9 and MMP-16 activities, and the inhibitory effect of n-butyl alcohol extract selectivity to MMP-9. Further experiments confirm N-butyl alcohol extract and final water extract also inhibit the migration of HT1080 cells. These results indicate the therapeutic mechanism of Ramulus Cinnamomi may involve the inhibition of MMPs.
matrix metalloproteinases Ramulus Cinnamomi traditional chinese medicines
Jingang Yang Pengda Sun Zhongyuan Wang Haili Ling Shuhua Zhao Franklin D.Shuler Dahai Yu Xuexun Fang
Key laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education,Jilin University Key laboratory of Molecular Enzymology and Enzyme Engineering of Ministry Education,Jilin University Department of Traditional Chinese medicine, China-Japan Union Hospital,Jilin University, Changchun, Department of Orthopaedics, West Virginia University, Morgantown, WV 26506, USA
国际会议
南京
英文
138-142
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）