Influence of hepatitis C virus genotypes and F protein on outcome of HCV infection among injection drug users
(0) The aim of the study was to investigate whether hepatitis C virus (HCV) genotypes and F protein influence the outcome of HCV infection among drug users. Total 168 injection drug users(IDUs) with HCV persistent infection and 192 IDUs with HCV self-limiting infection were investigated. HCV genotyping of IDUs with persistent infection was performed by using the Innolipa HCV Ⅱ line probe assay. Sera from IDUs with HCV self-limiting infection were tested for type-specific antibodies to HCV genotypes 1-6 by using the MUREX HCV Serotyping 1-6 Assay. The purified recombinant protein (HCV-F/GST) was coated onto microtiter plates as antigen. The secondary antibodies were horse radish peroxidase (HRP)-conjugated goat anti-human IgG. Sera of 360 IDUs were tested by indirect ELISA for detecting anti-HCV-F antibodies. There were significant differences in gender between the groups of IDUs with HCV persistent infection and those with HCV self-limiting infection (P =0.026). No differences in anti-HCV-F postive between the two groups were found, with the exception that anti-HCV-F postive in persistent infection was slightly higher than that in self-limiting infection (36. 3％ vs 27.6％). Univariate and multivariate analysis indicated that male(0R= 1. 43, 95％ CI = 1. 03~2. 49, P=0.032), HCV genotype 1 (OR=4. 18, 95％ CI=2. 14-8. 13, P=0.000 1) and HCV-F antibody (OR = 1. 73, 95％CI=1. 08~2. 47, P=0. 047) were independent risk factors for HCV persistent infection. Conclusion: The results indicate that besides HCV-F antibody, other factors including HCV genotype and sex also influence the outcome of HCV infection. This is the first study to show the association between HCV genotype, HCV F protein and the risk for HCV persistent infection among IDUs.
hepatitis C virus (HCV) genotypes F protein hcv persistent infection HCV selflimiting infection
Yun Zhang Jingjing Yang Haopeng Wang Xiaozhao Deng Rongbin Yu Zhongcan Wang Jie Wan Ke Xu
Institute of Militalry Medicine, Nanjing Command,Nanjing, 210002, China Department of Medical Management, Hohai University, Nanjing, 210024, China Department of Epidemiology and Biostatistics, School of Public Health,Nanjing Medical University, Na
国际会议
南京
英文
158-162
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）