Characterization of the functional domain of STT3a of oligosaccharyltransferase from Dunaliella salina
(0) To investigate the function of STT3a gene in salt adaptation of Dunaliella salina (D. salina), a pair of degenerate primers was designed according to conserved homologous amino acid sequences of VCVFTA and DVDYVL of STT3a from Chlamydomonas, Arabidopsis thaliana and other organisms. A cDNA sequence of 1 650 bp was amplified from D. salina by RT-PCR and 3 Rapid Amplification of cDNA Ends and encoded a whole functional domain of STT3a, which shared homology with Chlamydomonas (48％), Arabidopsis thaliana (50％), Homo sapiens (46％), etc. Real-time fluorescence quantitative PCR demonstrated that the STT3a mRNAs from D. salina were induced by increased concentration of NaCl, and increased to 11-fold higher by 3. 5 mol/L NaCl than that by 1. 5 mol/L NaCl (P<0. 01). In conclusion, the findings of this study demonstrate that the high expression of the STT3a gene enhances the capability of salt adaptation in D. salina.
Dunaliella salina STT3a gene salt adaptation
Cui Wang Jie Li Liping Liu Lei Zeng Lexun Xue
Laboratory for Cell Biology, Department of Biology, Zhengzhou University, Henan, 450001, China
国际会议
南京
英文
179-182
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）