Cloning and functional analysis of salt tolerance of a new member of RbcS gene family from Dunaliella Salina
Salt can inhibit photosynthesis in many plants, but it can enhance photosynthesis in Dunaliella Salina (D. salina). RbcS is very important in the CO2 assimilation and starch formation, but very little is known about its role in D. salina under the high salt stress. In order to study how photosynthesis is enhanced in D. salina grown under the high salinity, we cloned a new full-length cDNA of RbcS gene family from D. salina, and investigated its functions under light and salt tolerance in the current study. The results revealed that the transcription level of the RbcS2 induced by light was increased fast before a plateau was reached, and then decreased gradually. The m RNA expression of the RbcS2 under the high salinity (3. 0 mol/L NaCl) became higher than normal culture condition (1. 5 mol/L NaCl). It is concluded that the cloned sequence named RbcS2 is a new one of the RbcS gene family from D. salina, and its expression can be induced by light and high salinity.
Dunaliella salina Rbcs RACE salt tolerance functional analysis real-time fluorescent quantitative PCR
Jiuling Feng Yurong Chai Liuqing Cui Hongtao Liu Jie Li Lexun Xue
Laboratory for Cell Biology, Department of Biology
国际会议
南京
英文
183-186
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）