Construction of cDNA library of Dunaliella salina and cloning of the gene kinesin like calmodulin-binding protein
The aim in this study was to construct the cDNA library of Dunaliella salina and to clone a new gene kinesin like calmodulin-binding protein (KCBP). A total RNA of Dunaliella salina was isolated and purified. Subsequently, cDNA was synthesized from isolated mRNA and ligated into the pAP3neo predigested vector, which would be transformed into Escherichia coli DH10B by electrotransformation. The titers and recombinant rates were determined and the KCBP was screened from the cDNA library of Dunaliella salina by PCR. The results showed that the titer was 5. 6 × 106 pfu/mL, the percentage of recombination was about 90％, the sizes of most inserted fragments ranged between 0. 4 kb and 6. 0 kb with an average size of 1. 9 kb. It is concluded that a new gene KCBP, which belongs to kinesin-14 family, is cloned and successfully screened from the cDNA library of Dunaliella salina constructed in this study. The cDNA library will be contributed to further investigating the functional genomics of Dunaliella salina in the future.
Dunaliella salina Cdna library kinesin like calmodulin-binding protein
Liping Liu JieLi Cui Wang Yunmeng Yan Lexun Xue
Laboratory for Cell Biology, Department of Biology, Zhengzhou University, Henan, 450001, China
国际会议
南京
英文
196-199
2010-05-12（万方平台首次上网日期，不代表论文的发表时间）