Characterization and Heterologous Expression of a New Matrix Attachment Region Binding Protein from the Unicellular Green Alga Dunaliella salina

摘要：

Although interactions between the nuclear matrix and special regions of chromosomal DNA called matrix attachment regions (MARs) are implicated in various nuclear functions, the understanding of the regulation mechanism of MARs is still poor. A few MAR binding proteins have been isolated from some plants and animals, but not from the unicellular algae. Here we identify a novel MAR binding protein, DMBP-1, from the halotolerant alga Dunaliella salina. The cDNA of DMBP-1 is 2 322 bp long, which contains a 1 626 bp of an open reading frame encoding a polypeptide of 542 amino acids (59 kDa). Expressed DMBP-1 in E. coli specifically binds A/T-rich MAR DNA.

关键词： Dunaliella salina matrix attachment region MAR binding protein nuclear matrix

作者: Tian-yun Wang Lexun Xue Yafeng Wang Yingcai Feng Pengju Wang

作者单位: Laboratory for Cell Biology, Zhengzhou University, Zhengzhou, 450052, China ,Department of Biochemis Laboratory for Cell Biology, Zhengzhou University, Zhengzhou, 450052, China

会议类型: 国际会议

会议名称: International Symposium on Pharmacogenomics and the Regulation of Drug Metabolism Enzymes and Genes(药物基因组学和药物代谢酶及基因调控国际研讨会)

会议地点: 南京

会议语种:英文

页码: 209-211

在线出版日期: 2010-05-12（万方平台首次上网日期，不代表论文的发表时间）