Prediction and Identification of B-Cell Epitopes on Goose Parvovirus Non-structural Protein

摘要：

The non-structure (NS) gene of goose parvovirus (GPV) was translated into amino sequence by Editseq. The secondary structure and B-cell epitopes of the deduced protein were predicted by Protean. Combining the results according to these methods, the nonstructural protein (NSP) has complicated secondary structure. There are several potential B-cells epitopes on NSP. By means of overlapping peptides expressed in Escherichia coli in combination with Western blotting, B-cell epitopes were identified on NSP. The results show virtual linear immunodominant epitopes, which were found in C-terminal (amino acid residues 453-627).

关键词： goose parvovirus non-structure protein prediction identification epitope

作者: Tian-fei Yu Shu-li Shao Xing-jun Xu Ming Li Yuan Guo

作者单位: College of Life Science and Agriculture Forestry University of Qiqihar Qiqihar, Heilongjiang Provinc College of Computer and Control Engineering University of Qiqihar Qiqihar, Heilongjiang Province, Ch

会议类型: 国际会议

会议名称: 2011 International Conference on Medical Information and Bioengineering(2011年医药信息与生物工程国际会议 ICMIB 2011)

会议地点: 成都

会议语种:英文

页码: 93-97

在线出版日期: 2011-01-14（万方平台首次上网日期，不代表论文的发表时间）