Optimization of Cellulase Production by Trichoderma reesei HY07 Using Response Surface Methodology
(0) Response surface methodology (RSM) based on a threelevel, three-variable Box and Behnken factorial design (BBFD) was used to evaluate the interactive effects of corn stalk: bran ratio, tween80 and temperature on the cellulase production. The optimum conditions derived via RSM were: corn stalk: bran ratio 1.29:1, Tween80 H.OSuI and temperature 31 ℃ for carboxymethyl cellulase (CMCase) and corn stalk: bran ratio 0.77:1, Tween80 12.54μl and temperature 32℃ for filter paper activity (FPA). The actual experimental yield was 406.42 U/g for CMCase and 93.62U/g for FPA under optimum condition, which compared well to the maximum predicted value of 405.67 U/g and 91.29 U/g. The cellulase yield under optimal conditions was 1.45 fold for CMCase and 1.33 fold for FPA to the control.
cellulose solid state fermentation response surface methodology Carboxymethyl cellulase FPA
Shu Guowei Chen He Ji Liyuan
College of Life Science & Engineering, Shaanxi University of Science & Technology, Xian, China
国际会议
成都
英文
186-190
2011-01-14（万方平台首次上网日期，不代表论文的发表时间）