Quick and Sensitive Determination of Gene Expression of Fatty Acid Synthase by real-time PCR
Background: Obesity results from an imbalance between energy intake and energy expenditure, which leads to a pathological accumulation of adipose tissue, but the underlying mechanism at gene level is far from being elucidated. Methods: Pig primary adipocytes were cultured in vitro and the expression of Fatty Acid Synthase (FAS) was detected by real-time PCR. Results: By using the following formula, we were able to quantify the mRNA expression of FAS of unknown samples: Y=-3.156X + 41.21 (Y=threshold cycle, X=log starting quantity). The high concentrations of glucose group significantly improved the mRNA expression of FAS (P < 0.01) rather than 0.25％ and 0％ concentrations of glucose. Conclusion: Our results provide significant data for that confirm an association between different glucose level and FAS expression in preadipocytes.
Energy Lipid deposition Fatty Acid Synthase mRNA express Real-time-PCR
Zuo-Hua Liu Jin-Long Yang Fei-Yun Yang Ding-Biao Long Kang-Cheng Pan Dai-Wen Chen
Chongqing Academy of Animal Science, Chongqing Key Laboratory of Pig Industry Sciences 402460 Chongq College of Veterinary Medicine of Sichuan Agricultural University 625014 Yaan, Sichuan Province, Chi Institute of Animal Nutrition, Sichuan Agricultural University 625014 Yaan, Sichuan Province, China
国际会议
成都
英文
238-240
2011-01-14（万方平台首次上网日期，不代表论文的发表时间）