A Renewable Amperometric Immunosensor for hs-CRP Based on Functionalized Fe3O4@Au Magnetic Nanoparticles Attracted on Fe(III) Phthlocyanine/Chitosan-membrane Modified Screen-printed Carbon Electrode By A Magnet
A novel disposable screen-printed immunosensor for rapid determination of highly sensitive C reactiveprotein (hs-CRP) in human serum has been developed in the experiment The sensor was constructed on one screen-printed carbon electrode (SPCE) with HRP labeled anti-hs-CRP antibody functionalized Fe3O4@Au magnetic nanoparticles (HRP labeled anti hs-CRP/Fe3O4@Au) as the biorecognition probes attracted on the surface of Fe (III) phthalocyanine (FePc)/chitosan membrane modified screen-printed carbon electrode (SPCE|FePc/Chit/chitosan) by external magnetic field. FePc was acted as electron immediate. The modified electrode shows an excellent electrocatalytic activity for hs-CRP in phosphate buffer solution (pH=7.0). After the immunosensor is incubated with hs-CRP antigen solution at 37℃ for 20 min, the access of activity center of the HRP to electrode is partly inhibited, which leads to a linear decrease of the catalytic efficiency of the HRP to the reduction of immobilized FePc by H2O2 at -SO mV in hs-CRPs concentration ranges from 1.2 to 200 ng/mL. The detection limit was 0.5 ng/mL. The immunosensor was successfully utilized for determination of hs-CRP in real serum samples of heart disease patients, whose results were consistent with that by ELISA method. The accuracy and precision of the assay were 91.5104.4％ and 15.8-24.4％, respectively. The immunosensor was reusable once constructed and can be regenerated by adding new nanoprobes on the surface of basal electrode through magnet on its bottom. It can greatly reduce the detection cost which is valuable for the early diagnosis of tumors.
Renewable amperometric biosensor Fe(III) phthalocyanine megnetic Fe3O4/Au particles high sensitive C reactiveprotein
Ning Gan Linghua Meng Futao Hu Yuting Cao Yuanzhao Wu Liyong Jia Lei Zheng
Faculty of Materical Science and Chemical Engieering Ningbo University, Ningbo, P.R.China Nanfang Hospita, Guangzhou, 510515, China Southern Medical University Guangzhou, P.R.China
国际会议
2010 International Conference on Nano Science and Technology(2010年IEEE纳米科技国际会议 ICNST2010)
成都
英文
53-57
2010-12-17（万方平台首次上网日期，不代表论文的发表时间）