A new hydrogen peroxide biosensor based on HRP/Ag nanowires/glassy carbon electrode

摘要：

An amperometric biosensor for H2O2 detection was developed with immobilization of Horseradish Peroxidase (HRP) on Ag nanowires (AgNWs). Ag nanowires(AgNWs) have been prepared by the reductive deposition method and characterized by the scanning electron microscopy (SEM) and the energy dispersive X-ray (EDX). The electrochemical performance of the HRP/AgNWs/GC electrode was investigated by cyclic voltammetry (CV) and chronoamperometry and the use of Ag nanowires led to an efficient enzyme loading, and also provided an increased surface area for sensing the reaction, showing high electrocatalytic activity towards the reduction of H2O2. Under the optimized conditions, the response of the biosensor towards H2O2 was investigated by chronoamperometry. The biosensor exhibited excellent sensitivity (the detection limit was down to 0.005 mM), fast response time (15 sec). Moreover, the biosensor had long-time stability and good reproducibility.

关键词： Ag nanowire hydrogen peroxide biosensor Horseradish Peroxidase electrochemical method

作者: Min Zhang Faliang Cheng Zhiquan Cai Meiqiong Chen

作者单位: Dongguan University of Technology, Dongguan, Guangdong, 523106, China

会议类型: 国际会议

会议名称: 2010 International Conference on Nano Science and Technology(2010年IEEE纳米科技国际会议 ICNST2010)

会议地点: 成都

会议语种:英文

页码: 111-115

在线出版日期: 2010-12-17（万方平台首次上网日期，不代表论文的发表时间）