A molecular linkage map of sweetpotato based on SRAP markers
SRAP markers were applied to construct molecular genetic linkage maps of sweetpotato with 240 individuals of the F, population derived from a cross between sweetpotato Luoxushu 8 and Zheng-shu20. A total of 1 337 polymorphic molecular markers were generated with 344 SRAP primer combinations. There were 873 markers obtained in Luoxushu 8,which included 507 Simplex,212 Duplex,31 Triplex, and 123 Double-simplex intercross markers; 587 markers were obtained in Zhengshu 20, which included 356 Simplex, 104 Duplex,4 Triplex, 123 Double-simplex intercross and 85 distorted markers. U-sing the software of JoinMap 3. 0 and the double pseudo testcross strategy ,a molecular linkage map containing 90 linkage groups was constructed with 514 SRAP markers in Luoxushu 8,and the map coveed 6 720.03 cM with an average marker interval of 10.50 cM. In Zhengshu 20,the molecular linkage map containing 79 linkage groups was constructed using 384 SRAP markers, and the map covered 4 768.62 cM with an average marker interval of 12.42 cM. 11 and 12 homologous groups were identified by 104 and 28 multiple-dose markers in the Luoxushu 8 and Zhengshu 20 maps,respectively. There were 29 Double-simplex intercross markers present in paternal and maternal linkage maps,which allowed the identification of the 45 corresponding linkage groups between both parents.
sweetpotato SRAP marker molecular linkage map
Li Aixian Wang Qingmei Zhang Liming Zhang Haiyan Hou Fuyun Xie Beitao Liu Qingchang
Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong Crop Research Institute,Shandong Academyof Agricultural Sciences,Jinan 250100,Shandong China Agricultural University,Beijing 100193
国际会议
徐州
英文
45-55
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）