Analysis of genetic stability and diversity of sweetpotato germplasm with SSR
Genetic stability of 24 sweetpotato germplasms maintained in vitro more than 5 years in the National Sweetpotato in vitro Genebank in Xuzhou,was analyzed using Simple Sequence Repeat (SSR) molecular markers. 20 pairs SSR primers were used. The results showed that all sweetpotato germ-plasms stored in vitro had the same amplified products as the controls preserved in field nursery, indicating that the two different methods have the same effect on preserving sweetpotato germplasm stability. Meanwhile,we analyzed the genetic diversity of 24 sweetpotatoe germplasms. The dendrogram generated by UPMGA showed the genetic relationship among 24 accessions. The genetic similarity among 24 accessions varied from 0.57 to 0.93. The numbers of total bands and polymorphic bands generated by the 20 primer combinations were 30 and 21 respectively. The 24 accessions were divided into 3 groups by cluster analysis. These results may provide a theoretical basis for the breeding of sweetpotato.
sweet potato genetic stability genetic diversity SSR
Donglan Zhao Litao Zheng Jun Tang Zhilin Zhou Qinghe Cao
Xuzhou Sweetpotato Research Center,Jiangsu,China 221121 Guangdong Ocean University,Zhanjiang,Guangdong,China 524088
国际会议
徐州
英文
136-144
2010-11-27(万方平台首次上网日期,不代表论文的发表时间)