Isolation and characterization of sweetpotato IbOr gene in transgenic plants
Carotenoids are naturally occurring pigments in plants and other photosynthetic organisms. They are key elements of photosynthesis and protect plants from photodamage. In humans,carotenoids have vitamin A activity and can also act as antioxidants. Yellowfleshed sweetpotato Llpomoea batatas (L.) Lam. is famous for its rich contents of carotenoids as a functional food. We have previously cloned and characterized IbOr gene, which is responsible for biosynthesis and accumulation of carotenoids in chromoplast,from the storage roots of yellow-fleshed sweetpotato (cv. Shinhwangmi). In this study,we generated IbOr overexpressing Arabidopsis by Agrobacterium-mediated transformation. IbOr overexpression in transgenic plants was confirmed by RT-PCR and western blot analysis. The fresh weight of transgenic Arabidopsis seedlings was always higher than wild type (WT) seedlings. Transgenic plants showed enhanced germination rate,longer root length,and higher relative water contents on the media supplemented with NaCI compared to WT plants. Both chlorophyll and carotenoid contents in leaves of the transgenic Arabidopsis were increased by 20 to 50％ and by 10 to 30％ ,respectively. The expressions of NCEDs,AREB3 and ABF3,which are known to play important roles in ABA signaling and abiotic stress tolerance in plants,were significantly increased in IbOr overexpressing plants. Taken together, IbOr gene may be useful to develop transgenic plants with high contents of carotenoids on marginal lands.
sweetpotato β-carotene Orange gene salt stress ABA
AHN Young Ock KIM Sun Ha LEE Haeng-Soon KWAK Sang-Soo
Environmental Biotechnology Research Center,Korea Research Institute of Bioscience and Biotechnology (KRIBB),Daejeon 305-806,Korea
国际会议
徐州
英文
203-211
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）