The sweetpotato IbMYB1 gene as a potential visible marker for sweetpotato intragenic vector system
MYB transcription factors play important roles in transcriptional regulation of many secondary metabolites including anthocyanins. We cloned the R2R3-MYB type IbMYB1 cDNAs from the purple-fleshed sweetpotato (Ipomoea batatas L. cv Sinzami) and investigated the expression patterns of IbMYB1 gene with IbMYB1a and IbMYB1b splice variants in leaf and root tissues of various sweetpotato cultivars by RTPCR. The transcripts of IbMYB were predominantly expressed in the purple-fleshed storage roots and they were also detectable in the leaf tissues accumulating anthocyanin pigments. In addition,transcript levels of IbMYB1 gene were upregulated by treatment with methyl jasmonate (MJ) or salicylic acid (SA) in leaf and root tissues of cv. White Star. To set up the intragenic vector system in sweetpotato,we first evaluated the utilization of the IbMYB gene as a visible selectable marker. The IbMYB1a was transiently expressed in tobacco leaves under the control of a constitutive CaMV 35S promoter,a root-specific and sucrose-inducible SPO promoter,and an oxidative stress-inducible SWPA2 promoter. We also showed that overexpression of IbMYB1a induced massive anthocyanin pigmentation in tobacco leaves and upregulated the transcript levels of the structural genes in anthocyanin biosynthetic pathway. Furthermore,HPLC analysis revealed that the expression of IbMYB1a led to production of cy-anidin as a major core molecule of anthocyanidins in tobacco leaves. These results suggest that the IbMYB gene can be applicable to a visible marker for sweetpotato transformation with intragenic vectors,as well as the production of anthocyanin as important nutritive value in other plant species.
sweetpoatato slection marker anthocyanin intragenic vector
KIM Cha Young AHN Young Ock KIM Sun Ha KIM Yun-Hee LEE Haeng-Soon CATANACH Andrew S JACOBS Jeanne ME CONNER Anthony J KWAK Sang-Soo
Bioindustrial Process Center,Jeonbuk Branch Institute of Korea Research Institute of Bioscience and Environmental Biotechnology Research Center,Korea Research Institute of Bioscience and Biotechnology New Zealand Institute for Plant & Food Research Ltd, Private Bag 4704, Christchurch 8140, New Zealan
国际会议
徐州
英文
212-218
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）