Genetic modification of starch production in sweetpotato plant
To promote the utilization of sweetpotato starch,it is important to improve its productivity and physicochemical properties. Through the functional analyses of the genes expressed in storage roots, we found that overexpression of a Dof-type zinc finger transcription factor, SRF1, increased the starch content in the storage roots. Further molecular biological and biochemical analyses suggested that this increase of starch was likely to be caused through transcriptional suppression of vacuolar invertase. Also, molecular biological analyses of the cultivar Quick Sweet,which produces starch with much lower pasting temperature,suggested that isoform Ⅱ of starch synthase (SSⅡ) was involved in the regulation of pasting temperature. The transgenic plants with suppressed SSⅡ expression exhibited markedly lower pasting temperature of starch. These results suggest that transgenic approaches are useful in modifying the productivity and property of sweetpotato starch.
sweetpotato starch pasting temperature transformation genetic engineering
Masaru Tanaka Yasuhiro Takahata Motoyasu Otani Kenji Katayama Kanefumi Kitahara Osamu Nakayachi Hiroki Nakayama Masaru Yoshinaga
National Agricultural Research Center for Kyushu Okinawa Region, Yokoichi 6651-2,Miyakonojo,Miyazaki Research Institute for Bioresources and Biotechnology,Ishikawa Prefectural University,Suematsu 1-308 Faculty of Agriculture,Kagoshima University,Korimoto 1-21-24,Kagoshima 890-0065 Japan
国际会议
徐州
英文
226-230
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）