Tolerance to water stress in transgenic sweet potato expressing both SOD and APX
Two genotypes,Cu/Zn SOD and APX gene transferred sweet potato (TS) and non-transgenic sweet potato Upomoea batatas L. Yulmi) (NT) ,were used to study the drought tolerance in three different water stresses of 0,-0.44 MPa, - 0.78 MPa. Results showed that activities of Cu/Zn superox-ide dismutase (SOD) and ascorbate peroxidase (APX) increased under-0.44 MPa and decreased under - 0.78 MPa,net photosynthesis (Pn),stomatal conductance (Gs) and tissue water content decreased, intercellular CO-2 concentration (Ci) increased, then decreased under water stress,but under the same stress conditions,these indexes in the TS were always higher than in NT. The accumulation of hydrogen peroxide (H-2O-2) and superoxide anion radical (O~-2) increased the lipid peroxidation of the plasma membrane,prompted the accumulation of malondialdehyde (MDA),and the accumulation in the TS was always lower than the NT at the same PEG concentration. All these suggested that the transgenic sweet potato have stronger abilities to clean up active oxygen than the non-transgenic one, and can keep a higher tissue water content and Pn rate under water stress,expressing stronger drought resistant capability.
active oxygen Cu/Zn SOD APX transgenic sweet potato drought tolerance water stress Abbreviations
DENG Xi-ping XU Bing-cheng LI Yun KWAK Sang-soo
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau,Institute of Soil and Fourth Military Medical University,Xian 710032,China Korea Research Institute of Bioscience and Biotechnology,Daejeon 305-806,Korea
国际会议
徐州
英文
320-331
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）