Inhibition of metastasis and tumor growth by sweetpotato storage protein, sporamin
Previous studies have suggested that the Kunitz-type trypsin inhibitors (KTIs) have antitumor effect against a variety of malignant cells. Sporamin is a KTI that accounts for about 60％-80％ of total soluble proteins in sweetpotato roots. In the present study,the effect of intraperitoneal (i. p. ,50 mg/kg BW) and intragastric (i.g. ,3000 mg/kg BW) administration of sporamin for 9 days on the intraperitoneal xenografts of human ileocecal carcinoma (HCT-8) was tested in nude mice. The effect on the spontaneous lung metastasis of Lewis lung carcinoma (311) was tested after 25 days of treatment in C57BL/6 mice. The effect on the proliferation and invasiveness of human colorectal cancer SW480 cells was also tested in vitro. The number of tumor nodules,the volume of ascites and serum carcinoembryonic antigen level were significantly reduced by i.p. and i.g. administration of sporamin (p<0.01) in nude mice. The formation of lung metastasis colonies was also significantly reduced in C57BL/6 mice (p<0.05). Sporamin also significantly suppressed the proliferation,migration and invasion of SW480 cells in a dose-and time-dependent manner (p <0.05). Our findings demonstrate for the first time that sporamin,a natural protein isolated from sweetpotato, reduced the intraperitoneal growth of HCT-8 carcinoma cells in nude mice and inhibited the spontaneous pulmonary metastasis of Lewis lung carcinoma (311) cells in C57BL/6 mice and the proliferation,migration and invasion of SW480 cells in vitro.
sporamin sweetpotato cancer metastasis
Peng-Gao Li Tai-Hua Mu
Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Food Science and Technology, C Laboratory of Food Chemistry and Nutrition Science, Institute of Agro-Food Science and Technology, C
国际会议
徐州
英文
561-570
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）