Preparation and anti-hypertensive effect of the enzyme digest of sweet potato protein
About 60,000 tons of sweetpotato starch is produced in Japan. At the same time,about 30,000 tons of remnant, which can cause serious environmental problems, is discharged. Thus, we have researched the effective utilization of byproducts from the sweetpotato starch industry. In this paper,the preparation of an enzyme digest of sweetpotato protein with angiotensin l-converting enzyme (ACE) inhibitory activity from starch wastewater,as well as the antihypertensive effect on spontaneously hypertensive rats (SHR),is reported. Protein was prepared from sweetpotato starch wastewater by isoelec-tric focusing precipitation. Three kinds of proteases were selected for effective protein digestion. The digest,sweetpotato peptide (SPP),exhibited strong ACE inhibitory activity (IC50:18.2 μg/mL). SPP was orally administered by gavage to SHR at a dose of 100 mg/kg or 500 mg/kg. The systolic blood pressure and the diastolic blood pressure were measured at 0 (before administration),2,4,8,and 24 hours after administration. Oral administration of SPP showed a dose-dependent decrease in the systolic blood pressure in SHR. Significant differences between SPP-administered rats and control rats were recognized at 4 and 8 hours after administration in the 500 mg/kg-administered group and at 8 hours after administration in the 100 mg/kg-administered group. Diastolic blood pressure also decreased in the SPPadministered groups,although the difference between SPPadministered rats and control rats was not significant. These results indicate that SPP is a possible material for the prevention or treatment of hypertension.
sweetpotato peptide enzyme digests angiotensin l-converting enzyme spontaneously hypertensive rats hypertension
ISHIGURO Koji SAMESHIMA Yoto KUME Takashi IKEDA Ken-ichird MATSUMOTO Jun YOSHIMOTO Makoto
Crop Functionality and Utilization Research Team,National Agricultural Research Center for Kyushu Ok Kagoshima Prefectural Institute of Food Processing and Utilization,Kimpoucho-oono 2200,Minamisatuma,
国际会议
徐州
英文
581-586
2010-11-27（万方平台首次上网日期，不代表论文的发表时间）