Design of the Fluorescence Detector Device Based on Integrating Sphere

摘要：

The fluorescent detection device was designed based on LED and integrating sphere. System used monochromatic LED as the light source, which through the interference filter and integrating sphere of uniform radiation to reduce the influence of light on the sample. When the light source went through the PMT and follow-up circuit, the real-time PCR cycle fluorescence intensity was gotten, finally. This design could effectively reduce the volume of fluorescence detection device and improved the costeffective of instrument. The experiment was compared the results between the device and iCycler-iQ5 PCR instrument which excitation light source dose not have homogenization. The instrumental maximum deviations are 9.4％ and 26.0％. And the deviation of iCycler-iQS PCR instrument is 13.9％. Its obvious that the device have higher accuracy, better stability and reproducibility.

关键词： integrating sphere photomultiplier tube PCR fluorescence detection

作者: Qi Cheng Zihong Ye Ming Kong Xiaoping Yu

作者单位: College of Metrology & Measurement Engineering China Jiliang University Hangzhou, China College of Life Sciences China Jiliang University Hangzhou, China

会议类型: 国际会议

会议名称: 2010 International Conference on Measurement and Control Engineering(2010年IEEE测量与控制工程国际会议 ICMCE2010)

会议地点: 成都

会议语种:英文

页码: 365-368

在线出版日期: 2010-11-16（万方平台首次上网日期，不代表论文的发表时间）