Enzymatic Branched Assembly of P450cam with Putidaredoxin and Putidaredoxin Reductase Produces a Catalytically Competent Nanocatalyst

摘要：

Cytochrome P450s catalyze diverse types of reactions containing hydroxylations, epoxidations, dealkylations and heteroatom oxidations, which make P450s promising industrial catalysts. P450s require electrons from NAD(P)H to convert molecular oxygen into reactive species. However, excess amount of electron transfer partner proteins (more than several tens molar ratio of P450) is necessary to obtain sufficient reaction rate and coupling efficiency, and this is a bottleneck for practical application of P450s.

作者: Kentaro Ichiman Hidehiko Hirakawa

作者单位: Department of Bioengineering The University of Tokyo,Tokyo 113-8656,Japan Department of Chemistry and Biotechnology,The University of Tokyo,Tokyo 113-8656,Japan Center for Na

会议类型: 国际会议

会议名称: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)

会议地点: 成都

会议语种:英文

页码: 8-9

在线出版日期: 2010-11-05（万方平台首次上网日期，不代表论文的发表时间）