Study on oxidative pyrimidine metabolism in Rhodococcus erythropolis: Discovery and analysis of a novel midase,ureidomalonase

摘要：

In biological system, pyrimidines are metabolized through either reductive, oxidative, or pyridine utilization (rut) pathway, and so on (Fig. 1). We applied the oxidative pathway to enzymatic synthesis of 2-deoxyribonucleosides with purine bases from thymidine and purine bases by nucleoside phosphorylase. The introduction of oxidative pyrimidine degradation shifted the reaction equilibrium to nucleoside synthesis by removing a byproduct, thymine, and well improved the production yield. We elucidated the enzymes involved in the oxidative pathway in a bacterium Rhodococcus erythropolis JCM 3132, and clarified their physiological function.

作者: Chee-Leong Soon Sakayu Shimizu Jun Ogawa

作者单位: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University,Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan

会议类型: 国际会议

会议名称: The Eleventh China-Japan-Korea Joint Symposium on Enzyme Engineering(第十一届中日韩酶工程学术研讨会)

会议地点: 成都

会议语种:英文

页码: 25-26

在线出版日期: 2010-11-05（万方平台首次上网日期，不代表论文的发表时间）